Direct patterning of probe proteins on an antifouling PLL-g-dextran coating for reducing the background signal of fluorescent immunoassays - LAAS - Laboratoire d'Analyse et d'Architecture des Systèmes Access content directly
Journal Articles Biointerphases Year : 2013

Direct patterning of probe proteins on an antifouling PLL-g-dextran coating for reducing the background signal of fluorescent immunoassays

Abstract

The limit of detection of advanced immunoassays, biochips and micro/nano biodetection devices is impacted by the non-specific adsorption of target molecules at the sample surface. In this paper, we present a simple and versatile low cost method for generating active surfaces composed of antibodies arrays surrounded by an efficient anti-fouling layer, capable to decrease drastically the fluorescence background signal obtained after interaction with a solution to be analyzed. The technological process involves the direct micro-contact printing of the antibodies probe molecules on a pre-coated PLL-g-dextran thin layer obtained by contact printing using a flat PDMS stamp. Compared to other blocking strategies (ethanolamine blocking treatment, PLL-g-PEG incubation, PLL-g-dextran incubation, printing on a plasma-deposited PEO layer), our surface chemistry method is more efficient for reducing non-specific interactions responsible for a degraded signal/noise ratio.
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Dates and versions

hal-01268739 , version 1 (04-02-2016)
hal-01268739 , version 2 (28-05-2019)

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Amandine Egea, Emmanuelle Trévisiol, Christophe Vieu. Direct patterning of probe proteins on an antifouling PLL-g-dextran coating for reducing the background signal of fluorescent immunoassays. Biointerphases, 2013, 8, pp.37. ⟨10.1186/1559-4106-8-37⟩. ⟨hal-01268739v2⟩
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