%0 Journal Article %T Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation %+ Équipe Micro et nanosystèmes HyperFréquences Fluidiques (LAAS-MH2F) %+ Institut de pharmacologie et de biologie structurale (IPBS) %A Tamra, Amar %A Dubuc, David %A Rols, Marie-Pierre %A Grenier, Katia %< avec comité de lecture %Z Rapport LAAS n° 17028 %@ 0018-9480 %J IEEE Transactions on Microwave Theory and Techniques %I Institute of Electrical and Electronics Engineers %P 1 - 7 %8 2017 %D 2017 %R 10.1109/TMTT.2017.2653776 %K Biosensor %K dielectric spectroscopy %K electroporation %K microwave analysis %K single cell %Z Engineering Sciences [physics]/ElectromagnetismJournal articles %X This paper presents the monitoring of single cells subjected to electroporation using microwave dielectric spectroscopy. The experimental results showed first a clear distinction between two cell states: viable cells and affected ones by a chemical treatment (Saponin). It also pointed out a high correlation (R 2 > 0.94) with biological standard techniques in detecting the two types of electroporation: the reversible and irreversible ones. The developed microfluidic and microwave-based sensor exposes a decrease in the capacitive and conductive contrasts of the investigated single cells treated by irreversible electroporation indicating damages at the cellular level, while cells under reversible electroporation present a similar dielectric response to that of the nontreated cells. This result corresponds to results frequently employed in biological studies. More interestingly , a study of the kinetics of the cell's damage induction over time, by electroporation, has been experimentally done, which makes microwave dielectric spectroscopy an attractive technique for cell's electroporation researches. %G English %2 https://laas.hal.science/hal-01463946/document %2 https://laas.hal.science/hal-01463946/file/TMTT_Amar_2017.pdf %L hal-01463946 %U https://laas.hal.science/hal-01463946 %~ UNIV-TLSE2 %~ UNIV-TLSE3 %~ CNRS %~ INSA-TOULOUSE %~ LAAS %~ UT1-CAPITOLE %~ LAAS-MH2F %~ LAAS-MICRO-NANO-BIO-TECHNOLOGIES %~ INSA-GROUPE %~ IPBS %~ TOULOUSE-INP %~ UNIV-UT3 %~ UT3-INP %~ UT3-TOULOUSEINP