Multiparametric imaging of adhesive nanodomains at the surface of Candida albicans by atomic force microscopy - LAAS - Laboratoire d'Analyse et d'Architecture des Systèmes Access content directly
Journal Articles Nanomedicine: Nanotechnology, Biology and Medicine Year : 2015

Multiparametric imaging of adhesive nanodomains at the surface of Candida albicans by atomic force microscopy

Abstract

Candida albicans is an opportunistic pathogen. It adheres to mammalian cells through a variety of adhesins that interact with host ligands. The spatial organization of these adhesins on the cellular interface is however poorly understood, mainly because of the lack of an instrument able to track single molecules on single cells. In this context, the atomic force microscope (AFM) makes it possible to analyze the force signature of single proteins on single cells. The present study is dedicated to the mapping of the adhesive properties of C. albicans cells. We observed that the adhesins at the cell surface were organized in nanodomains composed of free or aggregated mannoproteins. This was demonstrated by the use of functionalized AFM tips and synthetic amyloid forming/disrupting peptides. This direct visualization of amyloids nanodomains will help in understanding the virulence factors of C. albicans. From the Clinical Editor: The present study reports on the successful atomic force microscopy-based mapping of the adhesive properties of C. albicans cells, an accomplishment that highlights the utility of AFM.
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Origin : Files produced by the author(s)
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)
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Dates and versions

hal-01553144 , version 1 (07-09-2017)

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Cécile Formosa-Dague, Marion L Schiavone, Anita Boisrame, Mathias L Richard, Raphael E Duval, et al.. Multiparametric imaging of adhesive nanodomains at the surface of Candida albicans by atomic force microscopy. Nanomedicine: Nanotechnology, Biology and Medicine, 2015, 11 (1), pp.57-65. ⟨10.1016/j.nano.2014.07.008⟩. ⟨hal-01553144⟩
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