%0 Journal Article %T Multiparametric imaging of adhesive nanodomains at the surface of Candida albicans by atomic force microscopy %+ Structure et Réactivité des Systèmes Moléculaires Complexes (SRSMC) %+ Équipe Ingénierie pour les sciences du vivant (LAAS-ELIA) %+ Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP) %+ MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) %A Formosa-Dague, Cécile %A Schiavone, Marion, L %A Boisrame, Anita %A Richard, Mathias, L %A Duval, Raphael, E %A Dague, Etienne %Z ANR [ANR-11-JSV5-001-01, SD 30024331]; "Direction Generale de l'Armement" (DGA); Lallemand SAS; Association Lorraine pour la Recherche et le Developpement de Composes Bioactifs %< avec comité de lecture %Z Rapport LAAS n° 15138 %@ 1549-9634 %J Nanomedicine: Nanotechnology, Biology and Medicine %I Elsevier %V 11 %N 1 %P 57-65 %8 2015 %D 2015 %R 10.1016/j.nano.2014.07.008 %M 25101882 %K Nanodomains %K Atomic force microscopy %K Adhesins %K Candida albicans %K Multiparametric AFM %Z Life Sciences [q-bio]/Microbiology and Parasitology/Mycology %Z Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] %Z Life Sciences [q-bio]/BioengineeringJournal articles %X Candida albicans is an opportunistic pathogen. It adheres to mammalian cells through a variety of adhesins that interact with host ligands. The spatial organization of these adhesins on the cellular interface is however poorly understood, mainly because of the lack of an instrument able to track single molecules on single cells. In this context, the atomic force microscope (AFM) makes it possible to analyze the force signature of single proteins on single cells. The present study is dedicated to the mapping of the adhesive properties of C. albicans cells. We observed that the adhesins at the cell surface were organized in nanodomains composed of free or aggregated mannoproteins. This was demonstrated by the use of functionalized AFM tips and synthetic amyloid forming/disrupting peptides. This direct visualization of amyloids nanodomains will help in understanding the virulence factors of C. albicans. From the Clinical Editor: The present study reports on the successful atomic force microscopy-based mapping of the adhesive properties of C. albicans cells, an accomplishment that highlights the utility of AFM. %G English %2 https://hal.laas.fr/hal-01553144/document %2 https://hal.laas.fr/hal-01553144/file/CF5-nanomedicine-revised_clean.pdf %L hal-01553144 %U https://hal.laas.fr/hal-01553144 %~ AGROPARISTECH %~ UNIV-TLSE2 %~ UNIV-TLSE3 %~ CNRS %~ INSA-TOULOUSE %~ INRA %~ LAAS %~ UT1-CAPITOLE %~ LAAS-MICRO-NANO-BIO-TECHNOLOGIES %~ UNIV-LORRAINE %~ INC-CNRS %~ LAAS-ELIA %~ UNIV-PARIS-SACLAY %~ AGREENIUM %~ AGROPARISTECH-SACLAY %~ INRA-SACLAY %~ AGROPARISTECH-ORG %~ AGROPARISTECH-SVS %~ INSA-GROUPE %~ TBI %~ INRAE %~ GS-BIOSPHERA %~ GS-HEALTH-DRUG-SCIENCES %~ INRAEOCCITANIETOULOUSE %~ MICALIS %~ TOULOUSE-INP %~ UNIV-UT3 %~ UT3-INP %~ UT3-TOULOUSEINP %~ TEST2-HALCNRS %~ TBI-PRISM %~ TBI-SCE