Rapid Discrimination Of Single-Nucleotide Differences Through The Spatial Analysis Of A Nanofluidic-Embedded Biosensor
Résumé
We demonstrate that the spatial analysis of nanofluidic-embedded biosensors permits the fast and direct discrimination of single-nucleotide difference (SND) within microRNA sequences in a single step interaction. We first show that the spatial hybridization profile depends on the duplex affinity, and we speculate that the affinity constant can be inferred during target injection, which is not possible in local analysis where the dissociation step through rinsing must be conducted. We finally manage to discriminate a GT mismatch on a microRNA sequence.
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