%0 Journal Article %T Working Together: Spatial Synchrony in the Force and Actin Dynamics of Podosome First Neighbors %+ École polytechnique (X) %+ Institut de pharmacologie et de biologie structurale (IPBS) %+ RainDance Technologies, Inc. %+ Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération (LBCMCP) %+ Laboratoire de Physique Théorique de la Matière Condensée (LPTMC) %+ Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC) %+ Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST) %+ Équipe Ingénierie pour les sciences du vivant (LAAS-ELIA) %A Proag, Amsha %A Bouissou, Anais %A Mangeat, Thomas %A Voituriez, Raphael %A Delobelle, Patrick %A Thibault, Christophe %A Vieu, Christophe %A Maridonneau-Parini, Isabelle %A Poincloux, Renaud %< avec comité de lecture %Z Rapport LAAS n°15241 %@ 1936-0851 %J ACS Nano %I American Chemical Society %V 9 %N 4 %P 3800 - 3813 %8 2015-02-18 %D 2015 %R 10.1021/nn506745r %Z Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] %Z Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]Journal articles %X Podosomes are mechanosensitive adhesion cell structures that are capable of applying protrusive forces onto the extracellular environment. We have recently developed a method dedicated to the evaluation of the nanoscale forces that podosomes generate to protrude into the extracellular matrix. It consists in measuring by atomic force microscopy (AFM) the nanometer deformations produced by macrophages on a compliant Formvar membrane and has been called protrusion force microscopy (PFM). Here we perform time-lapse PFM experiments and investigate spatial correlations of force dynamics between podosome pairs. We use an automated procedure based on finite element simulations that extends the analysis of PFM experimental data to take into account podosome architecture and organization. We show that protrusion force varies in a synchronous manner for podosome first neighbors, a result that correlates with phase synchrony of core F-actin temporal oscillations. This dynamic spatial coordination between podosomes suggests a short-range interaction that regulates their mechanical activity. %G English %L hal-01767608 %U https://laas.hal.science/hal-01767608 %~ X %~ UPMC %~ UNIV-TLSE2 %~ UNIV-TLSE3 %~ CNRS %~ UNIV-FCOMTE %~ UNIV-BM %~ INSA-TOULOUSE %~ ENSMM %~ LAAS %~ FEMTO-ST %~ X-DEP %~ UNIV-BM-THESE %~ LPTMC %~ UT1-CAPITOLE %~ LAAS-MICRO-NANO-BIO-TECHNOLOGIES %~ LAAS-ELIA %~ UNIV-PARIS-SACLAY %~ X-SACLAY %~ UPMC_POLE_2 %~ INSA-GROUPE %~ SORBONNE-UNIVERSITE %~ SU-SCIENCES %~ IPBS %~ ALLIANCE-SU %~ TOULOUSE-INP %~ UNIV-UT3 %~ UT3-INP %~ UT3-TOULOUSEINP %~ MCD %~ FRM