A Comparative Study of α-Hemolysin Expression in Supported Lipid Bilayers of Synthetic and Enriched Complex Bacterial Lipid - LAAS - Laboratoire d'Analyse et d'Architecture des Systèmes Access content directly
Journal Articles BioNanoScience Year : 2014

A Comparative Study of α-Hemolysin Expression in Supported Lipid Bilayers of Synthetic and Enriched Complex Bacterial Lipid

Abstract

The purpose of this short communication was to examine whether the formation of a supported lipid bilayer (SLB) made with purely synthetic lipid or with Escherichia coli complex lipid may have any influence on the production and incorporation of the transmembrane protein α-hemolysin from Staphylococcus. Different molar ratio of E. coli total extract lipid and synthetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were used to prepare SLB, which were characterized by combination of quartz crystal microbalance with dissipation monitoring (QCM-D), fluorescence recovery after photobleaching (FRAP), and atomic force microscopy (AFM). It was found that a 68:32 molar ratio was optimal to produce a SLB mimicking a bacterial lipid membrane. Comparing this later SLB with a purely synthetic SLB (100 % POPC) as receptacle for expression of the Staphylococcus α-hemolysin fused to eGFP by a cell-free expression system (CFES), we showed that both production and incorporation of this membrane protein was very similar.
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hal-01767672 , version 1 (16-04-2018)

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Angélique Coutable, Irina Randrianjatovo, Vincent Noireaux, Christophe Vieu, Christophe Thibault, et al.. A Comparative Study of α-Hemolysin Expression in Supported Lipid Bilayers of Synthetic and Enriched Complex Bacterial Lipid. BioNanoScience, 2014, 4 (2), pp.104 - 110. ⟨10.1007/s12668-014-0127-8⟩. ⟨hal-01767672⟩
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