Heterogeneity of cancer patients increases difficulties to find the best treatment for each patient. To adapt the treatment, the solution would be to detect the patient response in culture dish, instead to wait several months the result of the treatment measured by the disease regression. This would allow testing several treatments with different doses for the best response. Classical analytical techniques such as flow cytometry, confocal microscopy, immunohistochemistry, western blot, gene expression profiling, proteomics or metabolomics are not usually used for this kind of tests because of the heaviness of their implementation. High Frequency Dielectric Spectroscopy (HFDS) could be the solution while this approach compared to others presents several advantages: miniaturizing, processing speed, small sample, no invasive preparations such as fixation, no labeling and possible real time measurement. This opens the door to the development of HFDS for non-invasive biological analysis.