Microfluidics for minute DNA sample analysis: open challenges for genetic testing of cell-free circulating DNA in blood plasma - LAAS - Laboratoire d'Analyse et d'Architecture des Systèmes Access content directly
Journal Articles Micro and Nano Engineering Year : 2018

Microfluidics for minute DNA sample analysis: open challenges for genetic testing of cell-free circulating DNA in blood plasma

Abstract

Genetic testing based on the analysis of circulating cell-free DNA (cfDNA) in body fluids, especially blood plasma, is raising interest for the management and follow-up of many diseases, including cancer. Because the concentration of cfDNA is low and its composition mostly degraded, this material can only be assayed with the most sensitive nucleic acid processing technologies. cfDNA analysis therefore constitutes a model target and a driving force for innovation in microfluidic biotechnologies. Here, we overview the main physico-chemical characteristics of cfDNA, and provide a critical review on the different methods for its processing out of blood samples. Then, we describe recent microfluidic developments for high sensitivity DNA analysis, evaluate their practical relevance for cfDNA analysis, and identify a few challenges for technologists in the near future.

Dates and versions

hal-02338521 , version 1 (30-10-2019)

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Rémi Malbec, Jean Cacheux, Pierre Cordelier, Thierry Leichle, Pierre Joseph, et al.. Microfluidics for minute DNA sample analysis: open challenges for genetic testing of cell-free circulating DNA in blood plasma. Micro and Nano Engineering, 2018, 1, pp.25-32. ⟨10.1016/j.mne.2018.10.003⟩. ⟨hal-02338521⟩
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