Microcontact printing is a well-known patterning technique that uses polydimethylsiloxane (PDMS) stamps to print micro/nanoscale biomolecule patterns onto sensor surfaces, and is a popular technique to generate low cost protein or cell microarrays. Characterization of such biomolecule patterns is crucial for successful targeted applications. However, methods to perform quality control analysis of these patterns are limited owing to complexity in the analysis of micro-patterned monolayers of biomolecules over a large field of view. In this application note, we demonstrate the capability of SEEC Microscopy to provide rapid and efficient imaging based quality control analysis of microcontact printed fibronectin patterns over large surface areas with nanometric precision.