In this work, we present a new model for glucose detection using an Electrochemical Field Effect Transistor (ElecFET) with a MATLAB TM software. ElecFET is a device in which the basic element is a pH-ChemFET (pH-sensitive Chemical Field Effect Transistor) designed with an integrated platinum microelectrode around the sensitive gate dielectric capable to trigger electrochemical reactions. A number of parameters such as polarization time (T pol) and polarization voltage (Vp) have been investigated, as well as the enzymatic unit number per volume (Nenz), the Michaelis constant (K M) and the glucose concentration (C 0). Each of the aforementioned parameters influences the glucose sensor response. Experimental data compared with our numerical model showed a reasonable fit.