<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03025101</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-02T18:15:08+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Beyond the paradigm of nanomechanical measurements on cells using AFM: an automated methodology to rapidly analyse thousands of cells</title>
            <author role="aut">
              <persName>
                <forename type="first">S.</forename>
                <surname>Proa-Coronado</surname>
              </persName>
              <idno type="halauthorid">1675369-0</idno>
              <affiliation ref="#struct-103735"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Childérick</forename>
                <surname>Séverac</surname>
              </persName>
              <email type="md5">b2f86221a411c624e506758a654814b1</email>
              <email type="domain">cnrs.fr</email>
              <idno type="idhal" notation="string">childerick</idno>
              <idno type="idhal" notation="numeric">18595</idno>
              <idno type="halauthorid" notation="string">36954-18595</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-7267-6630</idno>
              <idno type="IDREF">https://www.idref.fr/135701791</idno>
              <affiliation ref="#struct-184934"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">A.</forename>
                <surname>Martinez-Rivas</surname>
              </persName>
              <idno type="halauthorid">1675370-0</idno>
              <affiliation ref="#struct-103735"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Etienne</forename>
                <surname>Dague</surname>
              </persName>
              <email type="md5">c499b1bdd4adaab42d64448e9cd7ce07</email>
              <email type="domain">laas.fr</email>
              <idno type="idhal" notation="string">etienne-dague</idno>
              <idno type="idhal" notation="numeric">15032</idno>
              <idno type="halauthorid" notation="string">35974-15032</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-3290-9166</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=uX-O1agAAAAJ&amp;hl=fr&amp;cstart=20&amp;pagesize=20</idno>
              <idno type="IDREF">https://www.idref.fr/074569627</idno>
              <affiliation ref="#struct-467299"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Etienne</forename>
                <surname>DAGUE</surname>
              </persName>
              <email type="md5">c499b1bdd4adaab42d64448e9cd7ce07</email>
              <email type="domain">laas.fr</email>
            </editor>
            <funder ref="#projanr-51849"/>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2020-11-26 10:43:30</date>
              <date type="whenModified">2025-10-22 18:04:08</date>
              <date type="whenReleased">2020-11-26 11:13:25</date>
              <date type="whenProduced">2020-01</date>
              <date type="whenEndEmbargoed">2020-11-26</date>
              <ref type="file" target="https://laas.hal.science/hal-03025101v1/document">
                <date notBefore="2020-11-26"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://laas.hal.science/hal-03025101v1/file/Autobiotip_clean.pdf" id="file-3025101-2664384">
                <date notBefore="2020-11-26"/>
              </ref>
              <ref type="externalLink" target="https://hal.archives-ouvertes.fr/hal-02321293/file/Autobiotip_clean.pdf"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="539345">
                <persName>
                  <forename>Etienne</forename>
                  <surname>DAGUE</surname>
                </persName>
                <email type="md5">c499b1bdd4adaab42d64448e9cd7ce07</email>
                <email type="domain">laas.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03025101</idno>
            <idno type="halUri">https://laas.hal.science/hal-03025101</idno>
            <idno type="halBibtex">proacoronado:hal-03025101</idno>
            <idno type="halRefHtml">&lt;i&gt;Nanoscale Horizons&lt;/i&gt;, 2020, 5 (1), pp.131-138. &lt;a target="_blank" href="https://dx.doi.org/10.1039/C9NH00438F"&gt;&amp;#x27E8;10.1039/C9NH00438F&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Nanoscale Horizons, 2020, 5 (1), pp.131-138. &amp;#x27E8;10.1039/C9NH00438F&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-3025101-2664384"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="UNIV-TLSE2">Université Toulouse 2</idno>
            <idno type="stamp" n="UNIV-TLSE3">Université de Toulouse</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="INSA-TOULOUSE">Institut National des Sciences Appliquées de Toulouse</idno>
            <idno type="stamp" n="LAAS">Laboratoire d'analyse et d'architecture des systèmes</idno>
            <idno type="stamp" n="UT1-CAPITOLE">Université Toulouse 1 Capitole</idno>
            <idno type="stamp" n="LAAS-MICRO-NANO-BIO-TECHNOLOGIES" corresp="LAAS">LAAS-Micro Nano Bio Technologies</idno>
            <idno type="stamp" n="LAAS-ELIA" corresp="LAAS-MICRO-NANO-BIO-TECHNOLOGIES">Équipe Ingénierie pour les sciences du vivant</idno>
            <idno type="stamp" n="INSA-GROUPE">Groupe INSA</idno>
            <idno type="stamp" n="TEST-HALCNRS">Collection test HAL CNRS</idno>
            <idno type="stamp" n="ANR">ANR</idno>
            <idno type="stamp" n="TOULOUSE-INP">Toulouse - INP</idno>
            <idno type="stamp" n="UNIV-UT3">Université Toulouse 3</idno>
            <idno type="stamp" n="UT3-INP">Université de Toulouse / Toulouse INP</idno>
            <idno type="stamp" n="UT3-TOULOUSEINP">Université de Toulouse / Toulouse INP</idno>
            <idno type="stamp" n="TEST7-HALCNRS">test 7 du portail hal cnrs</idno>
            <idno type="stamp" n="RENATECH">RENATECH</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="invited" n="0">No</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Beyond the paradigm of nanomechanical measurements on cells using AFM: an automated methodology to rapidly analyse thousands of cells</title>
                <author role="aut">
                  <persName>
                    <forename type="first">S.</forename>
                    <surname>Proa-Coronado</surname>
                  </persName>
                  <idno type="halauthorid">1675369-0</idno>
                  <affiliation ref="#struct-103735"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Childérick</forename>
                    <surname>Séverac</surname>
                  </persName>
                  <email type="md5">b2f86221a411c624e506758a654814b1</email>
                  <email type="domain">cnrs.fr</email>
                  <idno type="idhal" notation="string">childerick</idno>
                  <idno type="idhal" notation="numeric">18595</idno>
                  <idno type="halauthorid" notation="string">36954-18595</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-7267-6630</idno>
                  <idno type="IDREF">https://www.idref.fr/135701791</idno>
                  <affiliation ref="#struct-184934"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">A.</forename>
                    <surname>Martinez-Rivas</surname>
                  </persName>
                  <idno type="halauthorid">1675370-0</idno>
                  <affiliation ref="#struct-103735"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Etienne</forename>
                    <surname>Dague</surname>
                  </persName>
                  <email type="md5">c499b1bdd4adaab42d64448e9cd7ce07</email>
                  <email type="domain">laas.fr</email>
                  <idno type="idhal" notation="string">etienne-dague</idno>
                  <idno type="idhal" notation="numeric">15032</idno>
                  <idno type="halauthorid" notation="string">35974-15032</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-3290-9166</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=uX-O1agAAAAJ&amp;hl=fr&amp;cstart=20&amp;pagesize=20</idno>
                  <idno type="IDREF">https://www.idref.fr/074569627</idno>
                  <affiliation ref="#struct-467299"/>
                </author>
              </analytic>
              <monogr>
                <idno type="localRef">Rapport LAAS n° 20323</idno>
                <idno type="halJournalId" status="VALID">111913</idno>
                <idno type="issn">2055-6756</idno>
                <idno type="eissn">2055-6764</idno>
                <title level="j">Nanoscale Horizons</title>
                <imprint>
                  <publisher>Royal Society of Chemistry</publisher>
                  <biblScope unit="volume">5</biblScope>
                  <biblScope unit="issue">1</biblScope>
                  <biblScope unit="pp">131-138</biblScope>
                  <date type="datePub">2020-01</date>
                  <date type="dateEpub">2019-08</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1039/C9NH00438F</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="phys.meca.biom">Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph]</classCode>
              <classCode scheme="halDomain" n="phys.phys.phys-bio-ph">Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]</classCode>
              <classCode scheme="halDomain" n="phys.phys.phys-chem-ph">Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph]</classCode>
              <classCode scheme="halDomain" n="spi.nano">Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics</classCode>
              <classCode scheme="halDomain" n="sdv.mp">Life Sciences [q-bio]/Microbiology and Parasitology</classCode>
              <classCode scheme="halDomain" n="sdv.mp.bac">Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology</classCode>
              <classCode scheme="halDomain" n="sdv.mp.myc">Life Sciences [q-bio]/Microbiology and Parasitology/Mycology</classCode>
              <classCode scheme="halDomain" n="sdv.bc.ic">Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]</classCode>
              <classCode scheme="halDomain" n="sdv.bbm.bp">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Nanomechanical properties of cells could be considered as cellular biomarkers. The main method used to access the mechanical properties is based on nanoindentation measurements, performed with an operator manipulated Atomic Force Microscope (AFM) which is time-consuming and expensive. This is one of the reasons that prevent the transfer of AFM technology into clinical laboratories. In this paper we report a methodology which includes an algorithm (transferred to a script, executed on a commercial AFM) able to automatically move the tip onto a single cell and through several cells to record force curves combined with a smart strategy of cell immobilization. Cells are placed into microwells of a microstructured polydimethylsiloxane (PDMS) stamp. Inside a classical 100 × 100 μm2 AFM field, 100 cells can be immobilized. In an optimal configuration we were able to measure, within 4 h, a population of 900 Candida albicans cells both native and caspofungin treated, which represents an unprecedented performance. We discovered that the population is heterogeneous and can be divided, on the basis of nanomechanical properties, into 2 subgroups.</p>
            </abstract>
            <particDesc>
              <org type="ir" xml:id="fr-esr-fdr-ir-097">
                <orgName type="acronym">RENATECH+</orgName>
                <idno type="wikidata">Q33121910</idno>
              </org>
            </particDesc>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-103735" status="VALID">
          <orgName>Instituto Politecnico Nacional [Mexico]</orgName>
          <orgName type="acronym">IPN</orgName>
          <desc>
            <address>
              <addrLine>Av. Luis Enrique Erro S/N C.P. 07738, Ciudad de México, México</addrLine>
              <country key="MX"/>
            </address>
            <ref type="url">http://www.esimetic.ipn.mx/Paginas/Inicio.aspx</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-184934" status="OLD">
          <idno type="IdRef">233518266</idno>
          <idno type="RNSR">201320554P</idno>
          <orgName>Institut des Technologies Avancées en sciences du Vivant</orgName>
          <orgName type="acronym">ITAV</orgName>
          <date type="start">2013-01-01</date>
          <date type="end">2020-12-31</date>
          <desc>
            <address>
              <addrLine>Centre Pierre Potier, 1, Place Pierre Potier Oncopole entrée BBP 5062431106 Toulouse</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.itav.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-217752" type="direct"/>
            <relation active="#struct-443875" type="indirect"/>
            <relation name="UMS3039" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="researchteam" xml:id="struct-467299" status="OLD">
          <orgName>Équipe Ingénierie pour les sciences du vivant</orgName>
          <orgName type="acronym">LAAS-ELIA</orgName>
          <date type="end">2025-01-01</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://hal-laas.archives-ouvertes.fr/LAAS-ELIA/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-459" type="direct"/>
            <relation active="#struct-81148" type="indirect"/>
            <relation name="443875" active="#struct-443875" type="indirect"/>
            <relation active="#struct-116255" type="indirect"/>
            <relation active="#struct-301232" type="indirect"/>
            <relation active="#struct-443875" type="indirect"/>
            <relation active="#struct-116256" type="indirect"/>
            <relation active="#struct-217752" type="indirect"/>
            <relation name="UPR8001" active="#struct-441569" type="indirect"/>
            <relation active="#struct-448187" type="indirect"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-217752" status="OLD">
          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <idno type="ROR">https://ror.org/02v6kpv12</idno>
          <idno type="Wikidata">Q1273188</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <date type="end">2025-01-01</date>
          <desc>
            <address>
              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-443875" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-443875" status="VALID">
          <idno type="ROR">https://ror.org/017tgbk05</idno>
          <orgName>Communauté d'universités et établissements de Toulouse</orgName>
          <orgName type="acronym">Comue de Toulouse</orgName>
          <desc>
            <address>
              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.univ-toulouse.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-459" status="OLD">
          <idno type="IdRef">199278792</idno>
          <idno type="ISNI">0000 0001 2188 216X </idno>
          <idno type="RNSR">199517454Y</idno>
          <idno type="ROR">https://ror.org/03vcm6439</idno>
          <orgName>Laboratoire d'analyse et d'architecture des systèmes</orgName>
          <orgName type="acronym">LAAS</orgName>
          <date type="start">1968-01-01</date>
          <date type="end">2025-01-01</date>
          <desc>
            <address>
              <addrLine>7 Av du colonel Roche 31077 TOULOUSE CEDEX 4</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.laas.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-81148" type="direct"/>
            <relation name="443875" active="#struct-443875" type="indirect"/>
            <relation active="#struct-116255" type="direct"/>
            <relation active="#struct-301232" type="indirect"/>
            <relation active="#struct-443875" type="indirect"/>
            <relation active="#struct-116256" type="direct"/>
            <relation active="#struct-217752" type="direct"/>
            <relation name="UPR8001" active="#struct-441569" type="direct"/>
            <relation active="#struct-448187" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-81148" status="VALID">
          <idno type="IdRef">026404354</idno>
          <idno type="ISNI">0000000121902837</idno>
          <idno type="ROR">https://ror.org/0443n9e75</idno>
          <orgName>Université Toulouse Capitole</orgName>
          <orgName type="acronym">UT Capitole</orgName>
          <date type="start">1970-01-01</date>
          <desc>
            <address>
              <addrLine>2 rue du Doyen-Gabriel-Marty - 31042 Toulouse Cedex 9</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.ut-capitole.fr/</ref>
          </desc>
          <listRelation>
            <relation name="443875" active="#struct-443875" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-116255" status="VALID">
          <idno type="IdRef">026388766</idno>
          <idno type="ISNI">0000 0001 2286 8343</idno>
          <idno type="ROR">https://ror.org/01h8pf755</idno>
          <idno type="Wikidata">Q858979</idno>
          <orgName>Institut National des Sciences Appliquées - Toulouse</orgName>
          <orgName type="acronym">INSA Toulouse</orgName>
          <date type="start">1963-10-21</date>
          <desc>
            <address>
              <addrLine>135, avenue de Rangueil - 31077 Toulouse cedex 4</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.insa-toulouse.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-301232" type="direct"/>
            <relation active="#struct-443875" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-301232" status="VALID">
          <idno type="IdRef">162105150</idno>
          <orgName>Institut National des Sciences Appliquées</orgName>
          <orgName type="acronym">INSA</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="institution" xml:id="struct-116256" status="VALID">
          <idno type="IdRef">026403994</idno>
          <idno type="ROR">https://ror.org/04ezk3x31</idno>
          <orgName>Université Toulouse - Jean Jaurès</orgName>
          <orgName type="acronym">UT2J</orgName>
          <date type="start">1971-01-01</date>
          <desc>
            <address>
              <addrLine>5 allées Antonio Machado - 31058 Toulouse Cedex 9</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-tlse2.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-443875" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-448187" status="VALID">
          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <idno type="ROR">https://ror.org/033p9g875</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.inp-toulouse.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-443875" type="direct"/>
          </listRelation>
        </org>
      </listOrg>
      <listOrg type="projects">
        <org type="anrProject" xml:id="projanr-51849" status="VALID">
          <idno type="anr">ANR-20-CE42-0017</idno>
          <orgName>AutoBioTip</orgName>
          <desc>Automatisation des mesures mécanobiologiques par AFM, et de leur analyse par Apprentissage automatique</desc>
          <date type="start">2020</date>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>