%0 Journal Article %T Label-free detection of mitochondrial activity with Microwave Dielectric Spectroscopy Research Article %+ Équipe Micro et nanosystèmes HyperFréquences Fluidiques (LAAS-MH2F) %+ Centre de Recherches en Cancérologie de Toulouse (CRCT) %A Poiroux, Guillaume %A Dubuc, David %A Hosseini, Mohsen %A Artis, François %A Tamra, Amar %A Tosolini, Marie %A Pont, Frédéric %A Bosc, Claudie %A Saland, Estelle %A Lopez, Frédéric %A Fournié, Jean-Jacques %A Sarry, Jean-Emmanuel, E %A Grenier, Katia %A Poupot, Mary %< avec comité de lecture %Z Rapport LAAS n° 20329 %J International Journal of Biotechnology and Bioengineering %I World Academy of Science, Engineering and Technology %V 6 %N 5 %8 2020 %D 2020 %K mitochondria %K microwave dielectric spectroscopy %K apoptosis %K energetic metabolism %Z Life Sciences [q-bio]/Biotechnology %Z Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics %Z Engineering Sciences [physics]/ElectromagnetismJournal articles %X Mitochondrial bioenergetics contributes to important biological processes and its dysfunction underlies some diseases, but its assessment requires invasive methods involving intracellular staining and chemical inhibitors. In this study, we introduce microwave dielectric spectroscopy (MWDS) as a new non-invasive and label-free method to detect mitochondrial activity in live cells. We show that under electromagnetic radiation with microwaves (0.4-40 GHz), the dielectric properties of living cells are determined by their mitochondrial activity. MWDS instantly detects the mitochondrial depolarization induced by drugs targeting electron transport chain complexes or during the earliest events of the apoptotic process, from a cell suspension of a single microliter. MWDS also discriminates cancer cells with higher mitochondrial activity, an aspect often related to therapeutic resistance in cancer. Thereby, MWDS represents a highly innovative method for non-invasive detection of mitochondrial activity in live cells, with a broad range of applications in biology and medicine. %G English %2 https://hal.laas.fr/hal-03027924/document %2 https://hal.laas.fr/hal-03027924/file/IJBB%20Poiroux%202020.pdf %L hal-03027924 %U https://hal.laas.fr/hal-03027924 %~ INSERM %~ UNIV-TLSE2 %~ UNIV-TLSE3 %~ CNRS %~ INSA-TOULOUSE %~ LAAS %~ UT1-CAPITOLE %~ LAAS-MH2F %~ LAAS-MICRO-NANO-BIO-TECHNOLOGIES %~ INSA-GROUPE %~ TOULOUSE-INP %~ UNIV-UT3 %~ UT3-INP %~ UT3-TOULOUSEINP %~ CRCT