Detection of a Macromolecule Denaturation With Microwave Dielectric Spectroscopy based on Hydration Modifications - LAAS - Laboratoire d'Analyse et d'Architecture des Systèmes Access content directly
Conference Papers Year : 2020

Detection of a Macromolecule Denaturation With Microwave Dielectric Spectroscopy based on Hydration Modifications

Abstract

This paper focuses on demonstrating the possible detection with microwave dielectric spectroscopy of the hydration modification of a protein submitted or not to a chaotropic agent. The case study of the denaturation of the large BSA protein with urea is investigated. A hydration contrast is extracted from microwave measurements and presents a linear relationship with the concentration of the denaturing agent. This result demonstrates that microwave dielectric spectroscopy could contribute to evaluate intramolecular change of conformation (structuration/destructuration) of biomacromolecules based on hydration modifications.
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Dates and versions

hal-03253166 , version 1 (08-06-2021)

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Katia Grenier, Genevieve Pratviel, Hugo Mazur, David Dubuc. Detection of a Macromolecule Denaturation With Microwave Dielectric Spectroscopy based on Hydration Modifications. 2020 IEEE MTT-S International Microwave Biomedical Conference (IMBioC), Dec 2020, Toulouse (virtual), France. ⟨10.1109/IMBIoC47321.2020.9385022⟩. ⟨hal-03253166⟩
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