Single cell microwave biosensor for monitoring cellular response to electrochemotherapy - LAAS - Laboratoire d'Analyse et d'Architecture des Systèmes Access content directly
Journal Articles IEEE Transactions on Biomedical Engineering Year : 2022

Single cell microwave biosensor for monitoring cellular response to electrochemotherapy

Abstract

This paper presents a 40 GHz microwave biosensor used to monitor and characterize single cells (THP-1) subjected to electrochemotherapy and obtain an electronic signature of the treatment efficiency. This biosensor proposes a non-destructive and label-free technique that first allows, with the rapid measurement of single untreated cells in their culture medium, the extraction of two frequency-dependent dielectric parameters, the capacitance (C (f)) and the conductance (G (f)). Second, this technique can powerfully reveal the effects of a chemical membrane permeabilizing treatment (Saponin). At last, it permits us to detect, and predict, the potentiation of a molecule classically used in chemotherapy (Bleomycin) when combined with the application of electric pulses (principle of electrochemotherapy). Treatment-affected cells show a decrease in the capacitive and conductive contrasts, indicating damages at the cellular levels. Along with these results, classical biological tests are conducted. Statistical analysis points out a high correlation rate (R 2 >0.97), which clearly reveals the reliability and efficacy of our technique and makes it an attractive technique for biology related researches and personalized medicine.
Fichier principal
Vignette du fichier
TBME-Amar 2022 version HAL.pdf (1.72 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03713284 , version 1 (04-07-2022)

Identifiers

Cite

Amar Tamra, Amel Zedek, Marie-Pierre Rols, David Dubuc, Katia Grenier. Single cell microwave biosensor for monitoring cellular response to electrochemotherapy. IEEE Transactions on Biomedical Engineering, 2022, 69 (11), pp.3407 - 3414. ⟨10.1109/TBME.2022.3170267⟩. ⟨hal-03713284⟩
53 View
53 Download

Altmetric

Share

Gmail Facebook X LinkedIn More