To achieve better accuracy in their investigations, biologists have recently been using threedimensional models as intermediates between 2D cell culture and the in vivo study of tissues. Some of these models are based on spheroids, which are cellular aggregates retaining many characteristics of in vivo behaviors while being still easy to use and implement in labs. To study such objects, multiples observation techniques can be used according to the objective of the study, including those using electromagnetic waves as non-destructive, non-invasive and label free analysis. Low frequencies (<1MHz) ones are currently under investigation as Electrochemical Impedance Spectroscopy. However, unlike microwaves (from 300MHz to 60GHz), they cannot penetrate the cell. Furthermore, most of the devices dedicated to microwave dielectric characterization are only focusing on cellular scales lower than spheroids (single cell, cell mats or suspensions) or on tissues and organs. In this article, a microwave spectroscopy device using a coplanar waveguide (CPW) adapted to the study of spheroids is presented with the dielectric characterization of fixed spheroids, with capacitance and conductance measurements from 0.5 to 20 GHz, aiming at filling the scale gap in the state of the art.