Article Dans Une Revue Sensors and Actuators B: Chemical Année : 2023

Direct in situ and real-time visualization of salt-dependent thiolated-DNA immobilization and biosensing on gold in nanofluidic channels

Résumé

Thiol-gold (Au) conjugation is perhaps the most routinely applied surface chemistry approach to immobilize receptors within Au-based molecular sensing platforms. However, no previous studies have investigated in situ immobilization in a nanofluidic systems. In this work, flowthrough receptor immobilization is demonstrated, using a nanoslit induced depletion zone to enable direct real-time visualization for characterizing the effect of sodium chloride (NaCl) concentrations during immobilization of thiolated single-stranded DNA on Au. The results show that the amount of fixed-DNA on Au, as well as the non-specific adsorption of DNA on fused silica surface, strongly depend on NaCl levels. Furthermore, the non-specific interaction at high NaCl levels reduces the DNA available for immobilization on Au, but enriches the DNA molecules in the channel under wash by deionized water. By adding Tween-20 to decrease the non-specific adsorption and increasing the driving pressure to increase the DNA flux under high-salt level, the immobilization time can be further decreased to 10 min or less, which is at least an order of magnitude faster than recently proposed bioconjugation methods for thiolated-DNA–Au conjugation. Moreover, this work demonstrates the capability of the platform for DNA- and aptamer-based sensing assays, thereby opening up new strategies for developing biosensors using nanofluidic platforms.

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hal-04776649 , version 1 (11-11-2024)

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Deng-Kai Yang, Liang-Kun Yu, Chia-Tzu Kuo, Jui-Hong Weng, Thierry Leïchlé, et al.. Direct in situ and real-time visualization of salt-dependent thiolated-DNA immobilization and biosensing on gold in nanofluidic channels. Sensors and Actuators B: Chemical, 2023, 394, pp.134303. ⟨10.1016/j.snb.2023.134303⟩. ⟨hal-04776649⟩
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