Communication Dans Un Congrès Année : 2025

TOWARDS UNVEILING THE HIDDEN DYNAMICS OF BACTERIORHODOPSIN WITH MEMS-BASED ATOMIC FORCE MICROSCOPY

Résumé

Understanding membrane protein unfolding is essential for elucidating protein stability and function.

Here, we employ MEMS-based atomic force microscopy (AFM) to investigate the unfolding dynamics of bacteriorhodopsin (BR) with microsecond resolution. Compared to conventional cantilevers, High frequency MEMS probes minimize meniscus effects and enable highsensitivity force spectroscopy under dry conditions. Unfolding force-extension curves reveal discrete structural transitions consistent with BR domain unfolding. These findings demonstrate MEMS-AFM as a valuable tool for high-speed biomolecular force measurements. This study also lays the groundwork for implementing optomechanical probes at > 100 MHz, advancing timeresolved protein mechanics into the nanosecond regime.

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hal-05251930 , version 1 (12-09-2025)

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Suyambulingam Subramanian, Nicolas Mauran, Guillaume Jourdan, Ignacio Casuso, Bernard Legrand. TOWARDS UNVEILING THE HIDDEN DYNAMICS OF BACTERIORHODOPSIN WITH MEMS-BASED ATOMIC FORCE MICROSCOPY. 2025 23rd International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers), Jun 2025, Orlando, United States. pp.817-820, ⟨10.1109/Transducers61432.2025.11110349⟩. ⟨hal-05251930⟩
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