Regenerative potential of primary adult human neural stem cells on micropatterned bioimplants boosts motor recovery
Résumé
Background:
The adult brain is unable
to regenerate itself
sufficiently
after
large injuries
.
Therefore, hopes
rely o
n therapies using
neural stem cell or biomaterial
transplantation
to
sustain brain reconstruction. The aim
of the present
study was to
evaluate
the improvement
in
sensorimotor recovery brought about by
human
p
rimary adult NSCs
in combination with
bio
implant
s
.
Methods:
hNSCs were
pre
-
seeded
on implants
micropatterned
for neurite guidance
and
inserted
intracerebrally 2 weeks after
a primary motor cortex lesion in rats
. Long
-
term
behavio
u
r was significantly
improved
after
hNSC
-
Implants versus
cell
engraf
tment
in the grip
strength test.
MRI and immunohistological studies were conducted to elucidate
the
underlying
mechanisms of
neuro
implants integration
.
Results:
hNSC
-
I
mplants
promoted tissue
reconstruction
and
limited hemispheric atrophy and glial scar
expansion. After 3 months
,
grafted hNSCs
were
detected on
implants
and expressed mature neuron
al markers (NeuN,
MAP2, and SMI
312). They also migrated over a short distance
to the reconstructed and
to the
perilesional tissue
s
, where
26%
integrated as
mature
neurons.
Newly
formed
host neural
progenitors (
n
e
stin, DCX) colonized the implants, notably in the presence of hNSCs, and
participated to
tissue reconstruction. The microstructured bioimplants sustain
ed
the
guided
maturation of both grafted hNSCs and endo
genous progenitors.
Conclusions:
t
hese
immunohistological results are coherent
with
and could explain
the
late improvement observed
in sensorimotor recovery.
These findings provide
novel insight
s
into the regenerative potential
of primary adult hNSCs
combined with
microstructure
d
implants
.
Domaines
NeurobiologieOrigine | Fichiers produits par l'(les) auteur(s) |
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