Microwave dielectric spectroscopy of cell membrane permeabilization with saponin on human B lymphoma cells - LAAS - Laboratoire d'Analyse et d'Architecture des Systèmes Access content directly
Conference Papers Year : 2014

Microwave dielectric spectroscopy of cell membrane permeabilization with saponin on human B lymphoma cells

Abstract

This paper aims to contribute to the demonstration of the microwave dielectric spectroscopy relevance for biological applications. We demonstrate indeed that microwave dielectric spectroscopy may significantly identify the membrane permeabilization (and its consequence on cells) of cells induced by chemo-treatment. Standard deviations on both real and imaginary parts (resp.) of the permittivity between several experiments are below 0.08 and 0.06 (resp.), whereas the contrasts on these two parameters reach 1.92 and 1.30 (resp.) at 10 GHz. The contrasts to the standard deviation ratio are then above 22, which permits to convince the biologists that the technique can significantly and efficiently detect the viability state of a cells population in their traditional culture medium. Moreover, these results permits to predict that such a technique can evaluate the viability of a cells population with few percents of accuracy.
Fichier principal
Vignette du fichier
IMS2014 Francois_sapo 2.pdf (336.84 Ko) Télécharger le fichier
Origin Files produced by the author(s)
Loading...

Dates and versions

hal-01951717 , version 1 (11-12-2018)

Identifiers

  • HAL Id : hal-01951717 , version 1

Cite

François Artis, David Dubuc, Jean-Jacques Fournié, Mary Poupot, Katia Grenier. Microwave dielectric spectroscopy of cell membrane permeabilization with saponin on human B lymphoma cells. IEEE MTT-S International Microwave Symposium (IMS2014), Jun 2014, Tampa, United States. 4p. ⟨hal-01951717⟩
29 View
16 Download

Share

Gmail Mastodon Facebook X LinkedIn More