The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae - LAAS - Laboratoire d'Analyse et d'Architecture des Systèmes
Journal Articles eLife Year : 2021

The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae

Clara Bouyx
  • Function : Author
  • PersonId : 1129869
Marion Schiavone
  • Function : Author
  • PersonId : 1129870
Marie-Ange Teste
Anne Julien
  • Function : Author
Jean-Marie François

Abstract

Fungal adhesins (Als) or flocculins are family of cell surface proteins that mediate adhesion to diverse biotic and abiotic surfaces. A striking characteristic of Als proteins originally identified in the pathogenic Candida albicans is to form functional amyloids that mediate cis-interaction leading to the formation of adhesin nanodomains and trans-interaction between amyloid sequences of opposing cells. In this report, we show that flocculins encoded by FLO11 in Saccharomyces cerevisiae behave like adhesins in C. albicans. To do so, we show that the formation of nanodomains under an external physical force requires a threshold number of amyloid-forming sequences in the Flo11 protein. Then, using a genome editing approach, we constructed strains expressing variants of the Flo11 protein under the endogenous FLO11 promoter, leading to the demonstration that the loss of amyloid-forming sequences strongly reduces cell-cell interaction but has no effect on either plastic adherence or invasive growth in agar, both phenotypes being dependent on the N-and C-terminal ends of Flo11p. Finally, we show that the location of Flo11 is not altered either by the absence of amyloid-forming sequences or by the removal of the Nor C-terminus of the protein.
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Dates and versions

hal-03434693 , version 1 (18-11-2021)

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Clara Bouyx, Marion Schiavone, Marie-Ange Teste, Etienne Dague, Nathalie Sieczkowski, et al.. The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae. eLife, 2021, 10, pp.e68592. ⟨10.7554/eLife.68592⟩. ⟨hal-03434693⟩
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