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Journal Articles IEEE Transactions on Microwave Theory and Techniques Year : 2017

Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation

Abstract

This paper presents the monitoring of single cells subjected to electroporation using microwave dielectric spectroscopy. The experimental results showed first a clear distinction between two cell states: viable cells and affected ones by a chemical treatment (Saponin). It also pointed out a high correlation (R 2 > 0.94) with biological standard techniques in detecting the two types of electroporation: the reversible and irreversible ones. The developed microfluidic and microwave-based sensor exposes a decrease in the capacitive and conductive contrasts of the investigated single cells treated by irreversible electroporation indicating damages at the cellular level, while cells under reversible electroporation present a similar dielectric response to that of the nontreated cells. This result corresponds to results frequently employed in biological studies. More interestingly , a study of the kinetics of the cell's damage induction over time, by electroporation, has been experimentally done, which makes microwave dielectric spectroscopy an attractive technique for cell's electroporation researches.
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Dates and versions

hal-01463946 , version 1 (09-02-2017)

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Amar Tamra, David Dubuc, Marie-Pierre Rols, Katia Grenier. Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation. IEEE Transactions on Microwave Theory and Techniques, 2017, pp.1 - 7. ⟨10.1109/TMTT.2017.2653776⟩. ⟨hal-01463946⟩
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