Candida is one of the most common opportunistic fungal pathogens in humans. Its adhesion to the host cell is required in parasitic states, and is important for pathogenesis. Many studies have shown that there is an increased risk of developing candidiasis when normal tissue barriers are weakened or when immune defences are compromised for example during cancer treatment that induces immunosuppression. The mechanical properties of malignant cells, such as adhesiveness and viscoelasticity, which contribute to cellular invasion and migration are different from those of non-cancerous cells. To understand host invasion and its relationship with host cell health, we probed the interaction of Candida spp. with cancerous and non-cancerous human cell lines using atomic force microscopy in single cell force spectroscopy mode. There was significant adhesion between Candida and human cells, with more adhesion to cancerous versus non-cancerous cell lines. This increase in adhesion is related to the mechanobiological properties of cancer cells which have a disorganized cytoskeleton and lower rigidity. Altered geometry and cytoskeletal disruption of the human cells impacted adhesion parameters, underscoring the role of cytoskeletal organisation in Candida-human cell